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Kits, reagents, consumables, and other supplies for use in molecular biology procedures. Includes preparation, extraction, purification and labeling products for DNA, RNA, and nucleic acids.
MagNA Pure 96 disposables are nuclease free according to current Quality Control procedures. They are manufactured from material that is inert to chemical leaching at low and elevated temperatures, that could interfere with downstream applications.
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Deoxyribonuclease I (DNase I) is an endonucleaseisolatedfrom bovine pancreas that digests double and single stranded DNA into oligo and mononucleotides. Amplification Grade DNase I has been purified to remove RNase activity and is suitable for eliminating DNA from RNA preparations prior to sensitive applications such as RT-PCR (Reverse Transcriptase - Polymerase Chain Reaction). DNase I digests double- and single-stranded DNA into oligo- and mononucleotides. Using the Reaction Buffer provided DNA is removed from RNA preparations in a 15 minute digestion at room temperature. The DNase I is then inactivated by heating with the Stop SOLUTIONon. Heating also denatures hairpins in the RNA so the RNA can be used directly in reverse transcription.No current RNA isolation procedure removes 100% of the DNA. Many commercial DNase I formulations are contaminated with residual RNases. This RNase contamination can destroy or degrade valuable RNA samples prior to reverse transcription. Laborator
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BM purple is a chromogenic substrate for alkaline phosphatase (AP) designed for precipitating enzyme immunoassays. It develops a permanent dark purple band or spot at the AP binding site on the membrane or solid support. The stabilized substrate SOLUTIONon may be used directly from the bottle with no mixing or reconstitution. It gives less background staining than when using the BCIP/NBT two-bottle system without a loss of sensitivity. The reaction product has a dark purple color and is insoluble in water. The dark purple color can be visually seen.Appearance prior to use light yellow color After color development dark purple
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